Journal: Circulation: Heart Failure
Article Title: Decompensated Heart Failure Is Associated With Reduced Corin Levels and Decreased Cleavage of Pro–Atrial Natriuretic Peptide
doi: 10.1161/circheartfailure.109.895581
Figure Lengend Snippet: Figure 1. The corin-ANP system. Pro-ANP is cleaved by the serine protease corin to N-ANP and ANP.9 ANP activates natriuretic pep- tide receptor-A (NPR-A), a transmembrane guanylyl cyclase. NPR-A catalyzes the synthesis of cGMP, which mediates salt and water excretion, vasodilation, and other effects.19
Article Snippet: For the ELISA assay, wells of a flat-bottom microplate (R&D Systems) were precoated with the capture antihuman corin rat monoclonal antibody (4 g/mL PBS) produced against the recombinant extracellular domain of corin 67 to 1042 amino acids (R&D Systems).
Techniques: